Journal: STAR Protocols

Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

doi: 10.1016/j.xpro.2026.104513

Figure Lengend Snippet: Genomic DNA extraction Schematic flowchart of the Genomic DNA Extraction process. Schematic flowchart illustrating the major steps of genomic DNA extraction from cells using the Qiagen Blood & Cell Culture DNA Maxi Kit. The workflow includes cell lysis, protein digestion with Proteinase K, binding of genomic DNA to the column, washing steps, and elution of purified DNA. The final step yields high-quality, protein-free genomic DNA with an A260/A280 ratio of approximately 1.8, suitable for downstream fragmentation and PD-seq analysis.

Article Snippet: If Qiagen Blood & Cell Culture DNA Maxi Kit is unavailable, similar genomic DNA extraction kits from other suppliers (e.g., Promega Wizard Genomic DNA Purification Kit, Thermo Fisher GeneJET Genomic DNA Purification Kit, or equivalent products from other vendors) can be used with comparable buffer volumes and centrifugation conditions, following the manufacturer's instructions.

Techniques: DNA Extraction, Cell Culture, Lysis, Binding Assay, Purification